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western reserve strain  (ATCC)


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    Structured Review

    ATCC western reserve strain
    Western Reserve Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 190 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vaccinia+virus+western+reserve/Vaccinia+virus/pm42012649-30-13-21
    Average 96 stars, based on 190 article reviews
    western reserve strain - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Virus:

    Article Title: BiP/GRP78 is a pro-viral factor for diverse dsDNA viruses that promotes the survival and proliferation of cells upon KSHV infection
    Article Snippet: .. Vaccinia Virus Western Reserve (ATCC VR-1354) was expanded in HeLa cells and titrated in BSC1 cells. ..

    Article Title: BiP/GRP78 is a pro-viral factor for diverse dsDNA viruses that promotes the survival and proliferation of cells upon KSHV infection
    Article Snippet: To prevent viral DNA replication , these cells were induced with Dox in the presence of phosphonoformate (PFA) 100μM (Sigma Aldrich P6801). .. Vaccinia Virus Western Reserve (ATCC VR-1354) was expanded in HeLa cells and titrated in BSC1 cells. .. To determine the efficiency of KSHV DNA replication, DNA was isolated from BCBL-1-RTA at the indicated times following reactivation using the Dneasy blood and tissue kit following manufacturer guidelines (Qiagen 69581).

    Article Title: Antiviral activities of two nucleos(t)ide analogs against vaccinia, mpox, and cowpox viruses in primary human fibroblasts.
    Article Snippet: .. Vaccinia virus Western Reserve (WR, ATCC VR-1354) strain was propagated and purified by ultracentrifugation onto a sucrose cushion as described previously (Earl et al., 2001). ..

    Article Title: BiP/GRP78 is a pro-viral factor for diverse dsDNA viruses that promotes the survival and proliferation of cells upon KSHV infection.
    Article Snippet: .. Vaccinia Virus Western Reserve (ATCC VR-1354) was expanded in HeLa cells and titrated in BSC1 cells. ..

    Article Title: Antiviral activities of two nucleos(t)ide analogs against vaccinia, mpox, and cowpox viruses in primary human fibroblasts
    Article Snippet: .. Vaccinia virus Western Reserve (WR, ATCC VR-1354) strain was propagated and purified by ultracentrifugation onto a sucrose cushion as described previously ( ). ..

    Article Title: mRNA vaccines encoding fusion proteins of monkeypox virus antigens protect mice from vaccinia virus challenge.
    Article Snippet: The Vero cells and 293 T cells used in this study were originally obtained from American Type Culture Collection (ATCC) and cultured in complete Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillinstreptomycin. .. The Vaccinia virus Western Reserve (WR, VR-1354) was also obtained from ATCC and propagated in Vero cells. ..

    Article Title: Live attenuated smallpox vaccine candidate (KVAC103) efficiently induces protective immune responses in mice.
    Article Snippet: Spots were counted using an Immunospot reader (Cellular Technology Limited., Shaker Heights, OH, USA). .. To evaluate the protective effect of the vaccine candidate against viral infection in the mouse model, a median lethal dose (LD50) of the vaccinia virus Western Reserve (VACV-WR, ATCC, Cat# VR-1354) strain was tested. ..

    Article Title: mRNA vaccines encoding fusion proteins of monkeypox virus antigens protect mice from vaccinia virus challenge
    Article Snippet: The Vero cells and 293 T cells used in this study were originally obtained from American Type Culture Collection (ATCC) and cultured in complete Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. .. The Vaccinia virus Western Reserve (WR, VR-1354) was also obtained from ATCC and propagated in Vero cells. ..

    Western Blot:

    Article Title: BiP/GRP78 is a pro-viral factor for diverse dsDNA viruses that promotes the survival and proliferation of cells upon KSHV infection
    Article Snippet: .. Vaccinia Virus Western Reserve (ATCC VR-1354) was expanded in HeLa cells and titrated in BSC1 cells. ..

    Article Title: BiP/GRP78 is a pro-viral factor for diverse dsDNA viruses that promotes the survival and proliferation of cells upon KSHV infection
    Article Snippet: To prevent viral DNA replication , these cells were induced with Dox in the presence of phosphonoformate (PFA) 100μM (Sigma Aldrich P6801). .. Vaccinia Virus Western Reserve (ATCC VR-1354) was expanded in HeLa cells and titrated in BSC1 cells. .. To determine the efficiency of KSHV DNA replication, DNA was isolated from BCBL-1-RTA at the indicated times following reactivation using the Dneasy blood and tissue kit following manufacturer guidelines (Qiagen 69581).

    Article Title: Antiviral activities of two nucleos(t)ide analogs against vaccinia, mpox, and cowpox viruses in primary human fibroblasts.
    Article Snippet: .. Vaccinia virus Western Reserve (WR, ATCC VR-1354) strain was propagated and purified by ultracentrifugation onto a sucrose cushion as described previously (Earl et al., 2001). ..

    Article Title: BiP/GRP78 is a pro-viral factor for diverse dsDNA viruses that promotes the survival and proliferation of cells upon KSHV infection.
    Article Snippet: .. Vaccinia Virus Western Reserve (ATCC VR-1354) was expanded in HeLa cells and titrated in BSC1 cells. ..

    Article Title: Antiviral activities of two nucleos(t)ide analogs against vaccinia, mpox, and cowpox viruses in primary human fibroblasts
    Article Snippet: .. Vaccinia virus Western Reserve (WR, ATCC VR-1354) strain was propagated and purified by ultracentrifugation onto a sucrose cushion as described previously ( ). ..

    Article Title: mRNA vaccines encoding fusion proteins of monkeypox virus antigens protect mice from vaccinia virus challenge.
    Article Snippet: The Vero cells and 293 T cells used in this study were originally obtained from American Type Culture Collection (ATCC) and cultured in complete Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillinstreptomycin. .. The Vaccinia virus Western Reserve (WR, VR-1354) was also obtained from ATCC and propagated in Vero cells. ..

    Article Title: Live attenuated smallpox vaccine candidate (KVAC103) efficiently induces protective immune responses in mice.
    Article Snippet: Spots were counted using an Immunospot reader (Cellular Technology Limited., Shaker Heights, OH, USA). .. To evaluate the protective effect of the vaccine candidate against viral infection in the mouse model, a median lethal dose (LD50) of the vaccinia virus Western Reserve (VACV-WR, ATCC, Cat# VR-1354) strain was tested. ..

    Article Title: mRNA vaccines encoding fusion proteins of monkeypox virus antigens protect mice from vaccinia virus challenge
    Article Snippet: The Vero cells and 293 T cells used in this study were originally obtained from American Type Culture Collection (ATCC) and cultured in complete Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. .. The Vaccinia virus Western Reserve (WR, VR-1354) was also obtained from ATCC and propagated in Vero cells. ..

    Purification:

    Article Title: Antiviral activities of two nucleos(t)ide analogs against vaccinia, mpox, and cowpox viruses in primary human fibroblasts.
    Article Snippet: .. Vaccinia virus Western Reserve (WR, ATCC VR-1354) strain was propagated and purified by ultracentrifugation onto a sucrose cushion as described previously (Earl et al., 2001). ..

    Article Title: Antiviral activities of two nucleos(t)ide analogs against vaccinia, mpox, and cowpox viruses in primary human fibroblasts
    Article Snippet: .. Vaccinia virus Western Reserve (WR, ATCC VR-1354) strain was propagated and purified by ultracentrifugation onto a sucrose cushion as described previously ( ). ..

    Infection:

    Article Title: Live attenuated smallpox vaccine candidate (KVAC103) efficiently induces protective immune responses in mice.
    Article Snippet: Spots were counted using an Immunospot reader (Cellular Technology Limited., Shaker Heights, OH, USA). .. To evaluate the protective effect of the vaccine candidate against viral infection in the mouse model, a median lethal dose (LD50) of the vaccinia virus Western Reserve (VACV-WR, ATCC, Cat# VR-1354) strain was tested. ..



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